rabbit anti-gaba r β2 Search Results


adrenergic receptors  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology adrenergic receptors
Figure 5. Chronic stimulation counteracted the decrease of β2 receptor expression in dorsal horn after SNTR. a. Representative confocal images of β2 <t>adrenergic</t> receptor immunoreactivity in the spinal dorsal horn of naïve rats, and in the ipsilateral and contralateral dorsal horns at 14 dpi of SNTR, SNTR+rAES, SNTR+CES, and SNTR+iCES rats, with graphic representation of laminae I, II, and III as regions of interest considered for quantification. Scale bar 100 μm. b. Quantification of β2 immunoreactivity in the ipsilateral and contralateral dorsal horn of naïve, and SNTR rats compared with the electrical stimulation treatments at 14 and 21 dpi. *p < 0.05, **p < 0.01. [Color figure can be viewed at wileyonlinelibrary.com]
Adrenergic Receptors, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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triton x 100  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology triton x 100
Figure 5. Chronic stimulation counteracted the decrease of β2 receptor expression in dorsal horn after SNTR. a. Representative confocal images of β2 <t>adrenergic</t> receptor immunoreactivity in the spinal dorsal horn of naïve rats, and in the ipsilateral and contralateral dorsal horns at 14 dpi of SNTR, SNTR+rAES, SNTR+CES, and SNTR+iCES rats, with graphic representation of laminae I, II, and III as regions of interest considered for quantification. Scale bar 100 μm. b. Quantification of β2 immunoreactivity in the ipsilateral and contralateral dorsal horn of naïve, and SNTR rats compared with the electrical stimulation treatments at 14 and 21 dpi. *p < 0.05, **p < 0.01. [Color figure can be viewed at wileyonlinelibrary.com]
Triton X 100, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti na k atpase  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti na k atpase
FIGURE 1 Repeated injection of NE-100 in the NAc induces depressive-like behaviors. (A) The time course of the experimental procedure. (B) Schematic diagram of the NAc (left panel) and image of the NAc injected with Evans-blue (right panel). (C) The level of σ1R mRNA in the NAc was examined at the end of the behavioral tests (n = 8 mice per group; Student’s t-test). (D) Representative Western blots of biotin-labeled surface proteins (surface) and total proteins (total) σ1R in the NAc. Na+-K+ <t>ATPase</t> served as internal control and GAPDH served as a negative control. Bar graphs indicate the ratio of proteins at the cell surface to their total levels. **p < 0.01 vs. control mice (n = 8 mice per group; Student’s t-test). (E) Bar graphs show the distance traveled in OFT in control mice and NE-100 mice (n = 12 mice per group; Student’s t-test). (F,G) Bar graphs show the immobility time of FST and TST in control mice and NE-100 mice. **p < 0.01 vs. control mice (n = 11 mice in control group of TST and n = 12 mice in other groups; Student’s t-test).
Anti Na K Atpase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Chronic stimulation counteracted the decrease of β2 receptor expression in dorsal horn after SNTR. a. Representative confocal images of β2 adrenergic receptor immunoreactivity in the spinal dorsal horn of naïve rats, and in the ipsilateral and contralateral dorsal horns at 14 dpi of SNTR, SNTR+rAES, SNTR+CES, and SNTR+iCES rats, with graphic representation of laminae I, II, and III as regions of interest considered for quantification. Scale bar 100 μm. b. Quantification of β2 immunoreactivity in the ipsilateral and contralateral dorsal horn of naïve, and SNTR rats compared with the electrical stimulation treatments at 14 and 21 dpi. *p < 0.05, **p < 0.01. [Color figure can be viewed at wileyonlinelibrary.com]

Journal: Neuromodulation : journal of the International Neuromodulation Society

Article Title: Chronic electrical stimulation reduces hyperalgesia and associated spinal changes induced by peripheral nerve injury.

doi: 10.1111/ner.12927

Figure Lengend Snippet: Figure 5. Chronic stimulation counteracted the decrease of β2 receptor expression in dorsal horn after SNTR. a. Representative confocal images of β2 adrenergic receptor immunoreactivity in the spinal dorsal horn of naïve rats, and in the ipsilateral and contralateral dorsal horns at 14 dpi of SNTR, SNTR+rAES, SNTR+CES, and SNTR+iCES rats, with graphic representation of laminae I, II, and III as regions of interest considered for quantification. Scale bar 100 μm. b. Quantification of β2 immunoreactivity in the ipsilateral and contralateral dorsal horn of naïve, and SNTR rats compared with the electrical stimulation treatments at 14 and 21 dpi. *p < 0.05, **p < 0.01. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Sections were blocked with specific serum and incubated overnight with primary antibodies in 0.3% Triton X-100 in PBS, for staining microglial cells (anti-Iba1, goat, 1:200, Abcam (19,25)), astrocytes (anti-GFAP, rabbit, 1:500, DakoCytomation (25,26)), adrenergic receptors (anti-β2 receptors, rabbit, 1:500, Santa Cruz (13,27)), K+-Cl− cotransporter 2 (antiKCC2, rabbit, 1:500, Millipore (19)) and γ-aminobutyric acid (antiGABA, rabbit, 1:500, Sigma–Aldrich (28)).

Techniques: Expressing

FIGURE 1 Repeated injection of NE-100 in the NAc induces depressive-like behaviors. (A) The time course of the experimental procedure. (B) Schematic diagram of the NAc (left panel) and image of the NAc injected with Evans-blue (right panel). (C) The level of σ1R mRNA in the NAc was examined at the end of the behavioral tests (n = 8 mice per group; Student’s t-test). (D) Representative Western blots of biotin-labeled surface proteins (surface) and total proteins (total) σ1R in the NAc. Na+-K+ ATPase served as internal control and GAPDH served as a negative control. Bar graphs indicate the ratio of proteins at the cell surface to their total levels. **p < 0.01 vs. control mice (n = 8 mice per group; Student’s t-test). (E) Bar graphs show the distance traveled in OFT in control mice and NE-100 mice (n = 12 mice per group; Student’s t-test). (F,G) Bar graphs show the immobility time of FST and TST in control mice and NE-100 mice. **p < 0.01 vs. control mice (n = 11 mice in control group of TST and n = 12 mice in other groups; Student’s t-test).

Journal: Frontiers in molecular neuroscience

Article Title: Repeated inhibition of sigma-1 receptor suppresses GABA A receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors.

doi: 10.3389/fnmol.2022.959224

Figure Lengend Snippet: FIGURE 1 Repeated injection of NE-100 in the NAc induces depressive-like behaviors. (A) The time course of the experimental procedure. (B) Schematic diagram of the NAc (left panel) and image of the NAc injected with Evans-blue (right panel). (C) The level of σ1R mRNA in the NAc was examined at the end of the behavioral tests (n = 8 mice per group; Student’s t-test). (D) Representative Western blots of biotin-labeled surface proteins (surface) and total proteins (total) σ1R in the NAc. Na+-K+ ATPase served as internal control and GAPDH served as a negative control. Bar graphs indicate the ratio of proteins at the cell surface to their total levels. **p < 0.01 vs. control mice (n = 8 mice per group; Student’s t-test). (E) Bar graphs show the distance traveled in OFT in control mice and NE-100 mice (n = 12 mice per group; Student’s t-test). (F,G) Bar graphs show the immobility time of FST and TST in control mice and NE-100 mice. **p < 0.01 vs. control mice (n = 11 mice in control group of TST and n = 12 mice in other groups; Student’s t-test).

Article Snippet: Several antibodies were used in this study: anti-D2R (1:1,000; Millipore Cat#AB5084p, RRID:AB_2094980), anti-GABAAR-α1 (Sigma Cat#G4416, RRID:AB_477016), anti-GABAARα2(AbcamCat#ab72445, RRID:AB_1268929), anti-GABAAR-β2 (Affinity Biosciences Cat#DF6671, RRID:AB_2838633), antiGABAAR-β3 (Abcam Cat#ab98968, RRID:AB_10670809), anti-phosphorylation PKC (Epsilon Ser729; Abcam Cat#ab63387, RRID:AB_1142277), anti-PKC (Abcam Cat#ab253274, RRID:AB_2827663), anti-σ1R (B5; Santa Cruz Biotechnology Cat#sc-137075, RRID:AB_2285870), anti-Na+-K+ ATPase (Cell Signaling Technology Cat#3010, RRID:AB_2060983) and anti-GAPDH (Abcam Cat#ab181602, RRID:AB_2630358).

Techniques: Injection, Western Blot, Labeling, Control, Negative Control

FIGURE 3 Repeated injection of NE-100 suppresses GABAAR and D2R expression. (A) The levels of GABAAR-α1-5, -β1-3, -γ1-3, -δ subunits, and D1R, D2R mRNA in the NAc were examined at the end of the behavioral tests. *p < 0.05 vs. control mice (n = 8 mice per group; two-way ANOVA). (B) Bar graphs show levels of GABAAR-α1, GABAAR-α2, GABAAR-β2, and GABAAR-β3 protein in the NAc normalized by the level of GAPDH were normalized by control levels. *p < 0.05 vs. control mice (n = 8 mice per group; two-way ANOVA). (C) Representative Western blots of biotin- labeled surface proteins (surface) and total proteins (total) D2R in the NAc. Na+-K+ ATPase served as internal control and GAPDH served as a negative control. Bar graphs indicate the ratio of proteins at the cell surface to their total levels. **p < 0.01 vs. control mice (n = 8 mice per group; Student’s t -test).

Journal: Frontiers in molecular neuroscience

Article Title: Repeated inhibition of sigma-1 receptor suppresses GABA A receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors.

doi: 10.3389/fnmol.2022.959224

Figure Lengend Snippet: FIGURE 3 Repeated injection of NE-100 suppresses GABAAR and D2R expression. (A) The levels of GABAAR-α1-5, -β1-3, -γ1-3, -δ subunits, and D1R, D2R mRNA in the NAc were examined at the end of the behavioral tests. *p < 0.05 vs. control mice (n = 8 mice per group; two-way ANOVA). (B) Bar graphs show levels of GABAAR-α1, GABAAR-α2, GABAAR-β2, and GABAAR-β3 protein in the NAc normalized by the level of GAPDH were normalized by control levels. *p < 0.05 vs. control mice (n = 8 mice per group; two-way ANOVA). (C) Representative Western blots of biotin- labeled surface proteins (surface) and total proteins (total) D2R in the NAc. Na+-K+ ATPase served as internal control and GAPDH served as a negative control. Bar graphs indicate the ratio of proteins at the cell surface to their total levels. **p < 0.01 vs. control mice (n = 8 mice per group; Student’s t -test).

Article Snippet: Several antibodies were used in this study: anti-D2R (1:1,000; Millipore Cat#AB5084p, RRID:AB_2094980), anti-GABAAR-α1 (Sigma Cat#G4416, RRID:AB_477016), anti-GABAARα2(AbcamCat#ab72445, RRID:AB_1268929), anti-GABAAR-β2 (Affinity Biosciences Cat#DF6671, RRID:AB_2838633), antiGABAAR-β3 (Abcam Cat#ab98968, RRID:AB_10670809), anti-phosphorylation PKC (Epsilon Ser729; Abcam Cat#ab63387, RRID:AB_1142277), anti-PKC (Abcam Cat#ab253274, RRID:AB_2827663), anti-σ1R (B5; Santa Cruz Biotechnology Cat#sc-137075, RRID:AB_2285870), anti-Na+-K+ ATPase (Cell Signaling Technology Cat#3010, RRID:AB_2060983) and anti-GAPDH (Abcam Cat#ab181602, RRID:AB_2630358).

Techniques: Injection, Expressing, Control, Western Blot, Labeling, Negative Control